Tissue Culture Vessel Comparison
This table lists all major tissue culture vessels with their corresponding areas, ratios and volumes.
|Culture Vessel||Surface area (cm²)||Typical volume (mL)||Ratio to T-75||Ratio to 6-well plate|
|BD Falcon CultureSlide (per well)||0.7||0.5||0.0093||0.07|
|BD Falcon CultureSlide (per slide)||5.6||4||0.075||0.46|
|35 mm dish||10||2||0.13||1|
|60 mm dish||20||3.5||0.26||2|
|100 mm dish||60||10||0.80||6|
|150 mm dish||140||23||1.87||14|
These volumes are only a guide. In practice, volumes can and do vary substantially depending on the growth requirements of the culture in question. Volumes of up to 20 mL are normal and acceptable for a T-75 flask, for example. These larger volumes allow greater periods of continuous growth without nutrient depletion (e.g. over a weekend or other period).
First published on 25th June 2011 and last modified on 17th March 2014.